Alfa Laval - Culturefuge

Culturefuge

Culturefuge, özellikle memeli hücre kültürleri, mikrobiyal hücreler ve çökeltilmiş proteinler için geliştirilmiş benzersiz alttan beslemeli hermetik biyofarma separatörler (veya tam separasyo sistemleri) serisidir. Besin elde edilen nazik ve verimli hızlandırılması, hedef proteinlerin aşağı yönde saflaştırılmasını daha kolay ve daha uygun maliyetli hale getiren kesmeye duyarlı hücre duvarı zarlarının tahrip edilmesini önler.

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Özellikle hücre kültürleri için geliştirilmiş katı madde çıkaran separatörler

  • Tutarlı ürün kalitesi – tamamen hermetik koşullar, separatör hazne içindeki hava-sıvı arayüzünü etkin bir şekilde ortadan kaldırır
  • Azaltılmış kesme ve kontaminasyon riski ¬– benzersiz içi boş mil beslemesi, hücrelerin çok hassas bir şekilde ayrılmasını sağlar
  • Kolay ölçeklendirme – kompakt, esnek tasarım, pilot tesisten üretime doğru bir şekilde genişletilebilir
  • Maksimum hijyen – yıkama nozulları santrifüj haznesinin ve siklonun dışına ve çerçeve kapağının içine püskürtülür
  • Malzemeler proses için yasal gereklilikleri karşılar - CIP ve/veya SIP sistemlerini kullanarak otomatik temizleme ve sanitizasyon seçeneği

Culturefuge, hazır skide monte edilmiş olarak temin edilebilir ve ana basınçlı kap özelliklerini karşılar. Sistem versiyonları, servis ve proses sıvıları için boruları ve PLC ve pnömatik üniteli entegre elektrik sistemini içerir. Tasarım seçenekleri arasında çok ince elektro-cilalı ürün temas yüzeyi ve katı fazın taşınması için buharla sterilize edilebilir pompa bulunur.

Gentle product acceleration

The Culturefuge is a specially developed range of separators for gentle treatment of cell cultures and other shear-sensitive biological material. The unique hollow spindle concept enables gentle acceleration of the feed liquid to the rotational speed of the separator, thereby minimizing cell lysis. This prevents the release of unwanted intracellular proteins or minimizes the destruction of fragile protein precipitates. Yields are thus enhanced and downstream operations optimized.

Scalable cell culture separator

Ideal for cell culture fermentation and continuous cell harvesting under hermetic conditions, the Culturefuge separator delivers consistent product quality and can be accurately and reliably scaled up from use in pilot plants to full-scale production.

Airtight cell culture separator

The Culturefuge is a fully hermetic separator, which effectively eliminates any air-liquid interface inside the separator bowl. This prevents air entrainment, which can cause problems in the downstream filtration process. Furthermore, flow control devices can be located downstream thus enabling implementation of an obstruction-free, full-bore feed arrangement. 

Hygienic design

Culturefuge is engineered using materials that meet stringent regulatory requirements for processing. To maintain hygienic operating conditions, this cell culture centrifuge can be automatically cleaned and sanitized without requiring major disassembly or assembly using Sterilization-in-Place (SIP) and Cleaning-in-Place (CIP) systems. In addition, flushing nozzles spray outside the centrifuge bowl, cyclone and inside the frame hood for maximum hygiene.

Complete separation system

The Alfa Laval Culturefuge is supplied as a skid-mounted separation system consisting of a disk-stack separator mounted on a fixed base frame with horizontal drive shaft, worm gear, lubricating oil bath and hollow vertical bowl spindle, and piping for service liquids and process liquids. It includes an integrated electrical system with starter, programmable logic control (PLC) system and pneumatic unit.

The design of the cell culture separator is in accordance with most major pressure vessel specifications, including ASME and PED.

Design options

    • Superfine electro-polished product contact surface
    • Steam-sterilizable pump for transport of the solids phase
    • Delivery of skid-mounted unit for open operation, contained running, steam-sterilizable aseptic operation, or steam decontamination-only operation.

The Culturefuge range

  • Culturefuge 100 for pilot plants and small-scale production with capacities ranging from 200 to 2,000 l/hr.
  • Culturefuge 200 for medium scale production with capacities ranging from 1,000 to 5,000 l/hr.
  • Culturefuge 300 for medium to large scale production with capacities ranging from 2,000 to 8,000 l/hr.
  • Culturefuge 400 for large scale production with capacities ranging from 5,000 to
    20,000 l/hr.

Upon request, Alfa Laval can provide validation support services and a rigorous Factory Acceptance Test (FAT) in our in-house test facility.

Cuturefuge cell centrifuge - Benefits

  • Compact and flexible design
  • Sterilizable
  • CIP (Cleaning-In-Place)-able
  • Contained

The Culturefuge concept

Nasıl çalışır

The Culturefuge separator is designed to provide gentle fully hermetic treatment of cell cultures and other shear-sensitive biological materials. The biological material enters the cell centrifuge through a hollow spindle feed inlet and gradually accelerates as it moves upward to the disc stack, where the separation takes place in the rotating centrifuge bowl.  The separated liquid phase leaves through the liquid outlet at the top of the bowl. The collected solids in the solid space are intermittently discharged from the periphery of the bowl. During normal production the operating water keeps the sliding bowl bottom closed against the bowl hood. During discharge the sliding bowl bottom drops for a short time (less than a second) and the solids are ejected through the discharge ports.

Tek kullanımlık ilk yüksek kaliteli biyofarmösatik proses separatörleri

Yüksek kaliteli hücre kültürü ayırma proseslerinde endüstri standartlarını uzun süredir Alfa Laval separatörleri belirlemektedir. Yeni Alfa Laval CultureOne ile hassas işlem ve yüksek verim sağlayan tüm eşsiz yeniliklerimiz tek kullanımlık biyofarma proses çözümleri için artık mevcuttur.

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The Separator Innovator

Would you like to know more about our innovations in separation technology? Click on the link below. Alfa Laval invented the first disc stack separator and has led the way in the development of centrifuge technology for over a century. We are glad to share with you the expertise we have gained along the way. Visit our Separator Innovator knowledge base to learn more about advancements in separation and the steps Alfa Laval is taking to continue revolutionizing the technology.

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